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MICRO PROPAGATION
Micro propagation (tissue culture or invitro culture) refers to the multiplication of plants, in aseptic condition and in artificial growth medium from plant parts like meristem tip, callus, embryos anthers, axillary buds etc.
It is a method by which a true to type and disease free entire plant can be regenerated from a miniature piece of plant in aseptic condition in artificial growing medium rapidly throughout the year.
Merits of micro propagation:
It is a method by which a true to type and disease free entire plant can be regenerated from a miniature piece of plant in aseptic condition in artificial growing medium rapidly throughout the year.
Merits of micro propagation:
a) Tissue culture helps in rapid multiplication of true to type plants throughout the year.
b) A new plant can be regenerated from a miniature plant part, whereas, in conventional methods a shoot of considerable length is required.
c) Large number of plants can be produced in culture tubes in small space with uniform growth and productivity instead of growing them in large areas in nursery.
d) Plants raised by tissue culture are free from diseases.
e) Tissue culture coupled with somatic hybridization (production of hybrid cells by fusion of two protoplasts with different genetic makeup.)helps in evolving new cultivars in a short time.
f) Micro propagation facilitates long distance transport of propagation materials and long term storage of clonal materials.
g) Tissue culture methods are particularly effective in plants that don‘t breed true from seeds, seeds are not viable (male sterile) or not available (banana) and in plant where propagation by conventional methods are expensive (Orchids)
Demerits of Micro propagation:
a) The cost involved in setting up and maintenance of a laboratory is very high and may not justify their use in all the horticultural plants ordinarily.
b) Tissue culture techniques require skilled manpower.
c) Slight infection may damage the entire lot of plants.
d) Some genetic modification (mutation) of the plant may develop with some varieties and culture systems, which may alter the quality of the produce.
e) The seedlings grown under artificial condition may not survive when placed under normal environmental condition.
Methods of Micro propagation:
b) A new plant can be regenerated from a miniature plant part, whereas, in conventional methods a shoot of considerable length is required.
c) Large number of plants can be produced in culture tubes in small space with uniform growth and productivity instead of growing them in large areas in nursery.
d) Plants raised by tissue culture are free from diseases.
e) Tissue culture coupled with somatic hybridization (production of hybrid cells by fusion of two protoplasts with different genetic makeup.)helps in evolving new cultivars in a short time.
f) Micro propagation facilitates long distance transport of propagation materials and long term storage of clonal materials.
g) Tissue culture methods are particularly effective in plants that don‘t breed true from seeds, seeds are not viable (male sterile) or not available (banana) and in plant where propagation by conventional methods are expensive (Orchids)
Demerits of Micro propagation:
a) The cost involved in setting up and maintenance of a laboratory is very high and may not justify their use in all the horticultural plants ordinarily.
b) Tissue culture techniques require skilled manpower.
c) Slight infection may damage the entire lot of plants.
d) Some genetic modification (mutation) of the plant may develop with some varieties and culture systems, which may alter the quality of the produce.
e) The seedlings grown under artificial condition may not survive when placed under normal environmental condition.
Methods of Micro propagation:
Different methods of micro-propagation are Meristem culture, Callus culture, Cell culture, Embryo culture, Protoplast culture, Shoot apex grafting, and Pollen grain culture.
Invitro:
Invitro:
Latin for ‘‘in glass‖. Reactions, responses or experiments in an artificial environment in isolation from the whole organism.
In vivo: Latin for ―in living‖. Biological processes that occur with in the whole living organism.
In vivo: Latin for ―in living‖. Biological processes that occur with in the whole living organism.